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Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs)

Identifieur interne : 000722 ( Main/Exploration ); précédent : 000721; suivant : 000723

Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs)

Auteurs : Ewa L. Gregoraszczuk [Pologne] ; Katarzyna Milczarek [Pologne] ; Anna K. Wojtowicz [Pologne] ; Vidar Berg [Norvège] ; Janneche U. Skaare [Norvège] ; Erik Ropstad [Norvège]

Source :

RBID : Pascal:08-0106040

Descripteurs français

English descriptors

Abstract

This study investigated in vitro endocrine disrupting effects of three mixtures of POPS: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjøsa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48 h with different doses of 'Marine mix', 'Mjøsa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjøsa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix' > 'Mjøsa mix' > 'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.


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Le document en format XML

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<term>Animals (MeSH)</term>
<term>Apoptosis</term>
<term>Apoptosis (drug effects)</term>
<term>Caspase 3 (metabolism)</term>
<term>Cell Survival (drug effects)</term>
<term>Cell death</term>
<term>Cells, Cultured (MeSH)</term>
<term>Coculture Techniques (MeSH)</term>
<term>Cod Liver Oil (chemistry)</term>
<term>Cod Liver Oil (toxicity)</term>
<term>Dose-Response Relationship, Drug (MeSH)</term>
<term>Endocrine Disruptors (chemistry)</term>
<term>Endocrine Disruptors (isolation & purification)</term>
<term>Estradiol (metabolism)</term>
<term>Exposure</term>
<term>Female (MeSH)</term>
<term>Fish Proteins (metabolism)</term>
<term>Flame retardant</term>
<term>Follicular cell</term>
<term>Gadiformes (MeSH)</term>
<term>Gadus morhua (MeSH)</term>
<term>Gonadal Steroid Hormones (metabolism)</term>
<term>Granulosa Cells (drug effects)</term>
<term>Granulosa Cells (metabolism)</term>
<term>L-Lactate Dehydrogenase (metabolism)</term>
<term>Mixture</term>
<term>Norway (MeSH)</term>
<term>Ovarian follicle</term>
<term>Ovary</term>
<term>Persistent organic pollutant</term>
<term>Pesticides</term>
<term>Polychlorobiphenyls</term>
<term>Progesterone (metabolism)</term>
<term>Risk Assessment (MeSH)</term>
<term>Secretion</term>
<term>Steroid</term>
<term>Swine (MeSH)</term>
<term>Theca Cells (drug effects)</term>
<term>Theca Cells (metabolism)</term>
<term>Time Factors (MeSH)</term>
<term>Viability</term>
<term>Water Pollutants, Chemical (isolation & purification)</term>
<term>Water Pollutants, Chemical (toxicity)</term>
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<term>Apoptose (effets des médicaments et des substances chimiques)</term>
<term>Appréciation des risques (MeSH)</term>
<term>Caspase-3 (métabolisme)</term>
<term>Cellules cultivées (MeSH)</term>
<term>Cellules de la granulosa (effets des médicaments et des substances chimiques)</term>
<term>Cellules de la granulosa (métabolisme)</term>
<term>Cellules thécales (effets des médicaments et des substances chimiques)</term>
<term>Cellules thécales (métabolisme)</term>
<term>Facteurs temps (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Gadiformes (MeSH)</term>
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<term>Hormones sexuelles stéroïdiennes (métabolisme)</term>
<term>Huile de foie de morue (composition chimique)</term>
<term>Huile de foie de morue (toxicité)</term>
<term>L-Lactate dehydrogenase (métabolisme)</term>
<term>Norvège (MeSH)</term>
<term>Oestradiol (métabolisme)</term>
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<term>Perturbateurs endocriniens (isolement et purification)</term>
<term>Polluants chimiques de l'eau (isolement et purification)</term>
<term>Polluants chimiques de l'eau (toxicité)</term>
<term>Progestérone (métabolisme)</term>
<term>Protéines de poisson (métabolisme)</term>
<term>Relation dose-effet des médicaments (MeSH)</term>
<term>Suidae (MeSH)</term>
<term>Survie cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Techniques de coculture (MeSH)</term>
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<term>Water Pollutants, Chemical</term>
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<term>Norway</term>
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<term>Perturbateurs endocriniens</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en">
<term>Apoptosis</term>
<term>Cell Survival</term>
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<term>Theca Cells</term>
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<term>Apoptose</term>
<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Survie cellulaire</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
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<term>Theca Cells</term>
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<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Hormones sexuelles stéroïdiennes</term>
<term>L-Lactate dehydrogenase</term>
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<term>Coculture Techniques</term>
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<term>Time Factors</term>
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<term>Animaux</term>
<term>Appréciation des risques</term>
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<term>Gadiformes</term>
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<term>Norvège</term>
<term>Relation dose-effet des médicaments</term>
<term>Stéroïde</term>
<term>Suidae</term>
<term>Sécrétion</term>
<term>Exposition</term>
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<term>Cellule folliculaire</term>
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<term>Polluant organique persistant</term>
<term>Pesticide</term>
<term>Polychlorobiphényles</term>
<term>Retardateur flamme</term>
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<term>Mort cellulaire</term>
<term>Techniques de coculture</term>
<term>Viabilité</term>
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<term>Phényle éther(polybromo)</term>
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<front>
<div type="abstract" xml:lang="en">This study investigated in vitro endocrine disrupting effects of three mixtures of POPS: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjøsa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48 h with different doses of 'Marine mix', 'Mjøsa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjøsa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix' > 'Mjøsa mix' > 'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.</div>
</front>
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